Dilution and Solution Calculator
Six small calculators for everyday reagent work. Keep units consistent: both concentrations in the same unit, and both volumes in the same unit.
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For learning and checking your own calculations. Always follow your laboratory's SOPs and verified procedures.
Good to know
Dilution factor = total volume ÷ sample volume: 1 part sample + 9 parts diluent is a 1:10 dilution (factor 10). Multiply a diluted result by the dilution factor. Mass (g) = molarity × volume (L) × molecular weight. Normality = molarity × equivalents per mole (for example H₂SO₄ has 2). Molarity = (% w/v × 10) ÷ molecular weight.
Formula
C1 × V1 = C2 × V2
Dilution factor = (sample + diluent) ÷ sample
Serial dilution in tube n = step^n
Mass (g) = molarity × volume (L) × molecular weight
Normality = molarity × equivalents per mole
Molarity = (% w/v × 10) ÷ molecular weight
Common questions
How much stock do I need to make 50 mL of 10 mg/dL from 100 mg/dL?
C1V1 = C2V2: 100 × V1 = 10 × 50, so V1 = 5 mL of stock plus 45 mL of diluent.
What is the molarity of normal saline?
0.9% NaCl is about 0.154 M (0.9 × 10 ÷ 58.44).
What is the dilution in the sixth tube of a doubling series?
1:64 (2 to the power 6).
Sources
- Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics (solutions, osmolality, clearance)